研 究 業 績 （荒川）

1. 鈴木伸吾，福田怜，福士幸治，荒川圭太: 植物抽出物の過冷却促進活性の比較，低温生物工学会誌 63: 33-36（2017）

2. 川村浩平，嘉見大助，鈴木伸吾，田中大介，藤川清三，荒川圭太: ガラス化法による交雑ポプラの超低温保存，低温生物工学会誌 62: 123-126（2016）

3. 荒川圭太：樹木細胞の凍結挙動，低温環境の科学事典（河村公盛ほか編），朝倉書店（2016）

4. 桑原慎子, 鈴木伸吾, 藤川清三, 荒川圭太: 大腸菌で発現させた氷核タンパク質inaA の氷核活性について, 低温生物工学会誌 61: 131-134 (2015)

5. 鈴木伸吾, 高橋大輔, 桑原慎子, 上村松生 , 荒川圭太: 樹木の冬芽の自発休眠の解除期における可溶性タンパク質組成変化の分析, 低温生物工学会誌 60: 59-63 (2014)

6. Endoh, K., Kuwabara, C., Arakawa, K., Fujikawa, S.: Consideration of the reasons why dormant buds of trees have evolved extraorgan freezing as an adaptation for winter survival, Environmental and Experimental Botany, 106: 52-59 (2014)

7. Koyama T., Inada T., Kuwabara C., Arakawa K., Fujikawa S.: Anti-ice nucleating activity of polyphenol compounds against silver iodide, Cryobiology, 69: 223-228 (2014)

8. Kuwabara C., Terauchi R., Tochigi H., Takaoka H., Arakawa K., Fujikawa, S.: Analysis of supercooling activities of surfactants, Cryobiology, 69: 10-16 (2014)

9. Kuwabara, C., Wang, D., Endoh, K., Fukushi, Y., Arakawa, K., Fujikawa, S.: Analysis of supercooling activity of tannin-related polyphenols. Cryobiology, 67: 40-49 (2013)

10. 10.1016/j.cryobiol.2013.04.008

11. Kasuga, J., Endoh, K., Yoshiba, M., Taido, I., Arakawa, K., Uemura, M., Fujikawa, S.: Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees, Physiologia Plantarum, 148: 25-35 (2013) 10.1111/j.1399-3054.2012.01678.x

12. Wang D, Kuwabara C., Endoh K., Fukushi Y., Fujikawa S., Arakawa K.: Supercooling-facilitating hydrolyzable tannins isolated from xylem tissues of Cercidiphyllum japonicum. In Plant and Microbe Adaptations to Cold in a Changing World, Imai R., Yoshida M. Matsumoto N. (eds), Springer, New York, pp179-188 (2013)

13. 桑原慎子, Wang Donghui, 春日純, 森若元太, 荒川圭太, 小山寿恵, 稲田孝明, 藤川清三: 種々判定法による過冷却促進(氷核形成阻害)物質の効果の検討, 低温生物工学会誌, 58: 99-103

14. （2012）

15. 遠藤圭太, 岡田香織, 鈴木伸吾, 藤川清三, 荒川圭太, カツラ冬芽の細胞の凍結挙動, 低温生物工学会誌 58: 179-184 (2012)

16. Endoh, K., Fujikawa, S., Arakawa, K.: Freezing behavior of cells in evergreen needle leaves of fir (Abies sachalinensis), Cryobiology and Cryotechnology, 58: 125-134 (2012)

17. Kuwabara, C., Wang, D., Kasuga, J., Fukushi, Y., Arakawa, K., Koyama, T., Inada, T. Fujikawa, S.: Freezing activities of flavonoids in solutions containing different ice nucleators. Cryobiology, 64: 279-285 (2012) 10.1016/j.cryobiol.2012.02.012

18. Wang, D., Kasuga, J., Kuwabara, C., Endoh, K., Fukushi, Y., Fujikawa, S., Arakawa, K.: Presence of supercooling-facilitating (anti-ice nucleation) hydrolyzable tannins in deep supercooling xylem parenchyma cells in Cercidiphyllum japonicum. Planta, 235: 747-759 (2012) (10.1007/s00425-011-1536-3)

19. Bhyan, S.B., Minami, A., Kaneko, Y., Suzuki, S., Arakawa, K., Sakata Y. Takezawa D.: Cold acclimation in the moss Physcomitrella patens involves abscisic acid-dependent signaling. Journal of Plant Physiology, 169: 137-145 (2012) 10.1016/j.jplph.2011.08.004

20. Endoh, K., Fujikawa, S., Arakawa, K.: Freezing behavior of cells in evergreen needle leaves of fir (Abies sachalinensis), Cryobiology and Cryotechnology, 58: 125-134 (2012)

21. Wang, D., Kasuga, J., Kuwabara, C., Fukushi, Y., Fujikawa, S., Arakawa, K.: Characterization of supercooling-facilitating (anti-ice nucleation) hydrolysable gallotannins in supercooling xylem parenchyma cells in katsura tree (Cercidiphyllum japonicum). Cryobiology and Cryotechnology, 57: 147-151 (2011)

22. Kuwabara, C., Kasuga, J., Wang, D., Fukushi, Y., Arakawa, K., Koyama, T., Inada, T., Fujikawa, S.: Change of supercooling capability in solutions containing different kinds of ice nucleators by flavonol glycosides from deep supercooling xylem parenchyma cells in trees. Cryobiology, 63: 157-163 (2011) 10.1016/j.cryobiol.2011.06.001

23. 荒川圭太，藤川清三：第3 章 3. 環境ストレスと形成層活動, 木質の構造（日本木材学会編）, 文永堂出版, p.134-139 (2011)

24. Umebayashi, T., Utsumi, Y., Koga, S., Inoue, S., Matsumura, J., Oda, K., Fujikawa, S., Arakawa, K., Otsuki, K.: Xylem water-conducting patterns of 34 broadleaved evergreen trees in southern Japan. Trees, 24: 571-583 (2010) 10.1007/s00468-010-0428-7

25. Kasuga, J., Fukushi, Y., Kuwabara, C., Wang, D.H., Nishioka, A., Fujikawa, E., Arakawa, K., Fujikawa, S.: Analysis of supercooling-facilitating (anti-ice nucleation) activity of flavonol glycosides. Cryobiology, 60, 240-243 (2010) 10.1016/j.cryobiol.2009.12.004

26. Ukaji, N., Kuwabara, C., Kanno, Y., Seo, M., Takezawa, D., Arakawa, K., Fujikawa, S.: Endoplasmic reticulum-localized small heat shock protein that accumulates in mulberry tree (Morus bombycis Koidz.) during seasonal cold acclimation is responsive to abscisic acid. Tree Physiology, 30: 502-513 (2010) 10.1093/treephys/tpp125

27. Tsutsui, T., Kato, W., Asada, Y., Sako, K., Sato, T., Sonoda, Y., Kidokoro, S., Yamaguchi-Shinozaki, K., Tamaoki, M., Arakawa, K., Ichikawa, T., Nakazawa, M., Seki, M., Shinozaki, K., Matsui, M., Ikeda, A., Yamaguchi, J.: DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis. Journal of Plant Research, 122: 633-643 (2009) 10.1007/s10265-009-0252-6

28. Endoh, K., Kasuga, J., Arakawa, K., Ito, T., Fujikawa, S.: Cryo-scanning electron microscopic study on freezing behaviors of tissue cells in dormant buds of larch (Larix kaempferi). Cryobiology, 59: 214-222 (2009) 10.1016/j.cryobiol.2009.07.010

29. Arakawa K., Inada H., Fujikawa, S.: Impact of simulated acid snow stress on leaves of cold-acclimated winter wheat. In Plant Cold Hardiness -From the laboratory to the field- Gusta, L.V., Wisniewski, M.E., Tanino, K.K. (eds), CAB International, UK, pp 173-182 (2009)

30. Fujikawa, S., Kasuga, J., Takata, N., Arakawa, K.: Factors related to change of deep supercooling capability in xylem parenchyma cells of trees. In Plant Cold Hardiness -From the laboratory to the field-, Gusta, L.V., Wisniewski, M.E., Tanino, K.K. (eds), CAB International, UK, pp 29-42 (2009)

31. 稲田秀俊, 藤川清三, 荒川圭太: 凍結過程に存在する酸性物質が植物細胞の生存に及ぼす影響.日本冷凍空調学会論文集, 25: 233-238 (2008)

32. Kami, D., Kasuga, J., Arakawa, K., Fujikawa, S.: Improved cryopreservation by diluted vitrification solution with supercooling-facilitating flavonol glycoside. Cryobiology, 57:242-245 (2008) 10.1016/j.cryobiol.2008.09.003

33. Kasuga, J., Hashidoko, Y., Nishioka, A., Yoshiba, M., Arakawa, K., Fujikawa, S.: Deep supercooling xylem parenchyma cells of katsura tree (Cercidiphyllum japonicum) contain flavonol glycosides exhibiting high anti-ice nucleation activity. Plant, Cell and Environment, 31: 1335-1348 (2008) 10.1111/j.1365-3040.2008.01835.x

34. Umebayashi, T., Utsumi, Y., Koga, S., Inoue, S., Fujikawa, S., Arakawa, K., Matsumura, J., Oda, K.: Conducting pathways in north temperate deciduous broadleaved trees. IAWA Journal, 29: 247-263 (2008)

35. Nagao, M., Arakawa, K., Takezawa, D., Fujikawa, S.: Long- and short-term freezing induce different types of injury in Arabidopsis thaliana leaf cells. Planta, 227: 477-489 (2008)

36. Kasuga, J., Mizuno, K., Arakawa, K., Fujikawa, S.: Anti-ice nucleation activity in xylem extracts from trees that contain deep supercooling xylem parenchyma cells. Cryobiology, 55: 305-314 (2007)

37. Takata, N., Kasuga, J., Takezawa, D., Arakawa, K., Fujikawa, S.: Gene expression associated with increased supercooling capability in xylem parenchyma cells of larch (Larix kaempferi). Journal of Experimental Botany, 58: 3731-3742 (2007)

38. Umebayashi, T., Utsumi, Y., Koga, S., Inoue, S., Shiiba, Y., Arakawa, K., Matsumura, J., Oda, K.: Optimal conditions for visualizing water-conducting pathways in a living tree by the dye injection method. Tree Physiology, 27: 993-999 (2007)

39. Kasuga, J., Takata, N., Yamane, K., Kuroda, K., Arakawa, K., Fujikawa, S.: Larch (Larix kaempferi) xylem parenchyma cells respond to subfreezing temperature by deep supercooling. CryoLetters, 28: 77-81 (2007)

40. Kasuga J., Arakawa, K., Fujikawa, S.: High accumulation of soluble sugars in deep supercooling Japanese white birch xylem parenchyma cells. New Phytologist，174: 569-579 (2007)

41. Inada, H., Fujikawa, S., Saito H., Arakawa, K.: Effects of light condition after simulated acid snow stress on leaves of winter wheat. Environmental Sciences, 14, 53-71 (2007)

42. 稲田秀俊, 藤川清三, 荒川圭太: 酸性雪ストレスに対する越冬性植物の生理応答， 低温生物工学会誌. 52: 163-168 (2006)

43. Kasuga, J., Mizuno, K., Miyaji, N., Arakawa, K., Fujikawa, S.: Role of intracellular contents to facilitate supercooling capability in beech (Fagus crenata) xylem parenchyma cells. CryoLetters, 27: 305-310 (2006)

44. Nagao, M., Oku K., Minami A., Mizuno K., Sakurai M., Arakawa, K., Fujikawa, S., Takezawa D.: Accumulation of theanderose in association with development of freezing tolerance in the moss Physcomitrella patens. Phytochemistry, 67: 702-709 (2006)

45. Fujikawa, S., Ukaji, N., Nagao, M., Yamane, K., Takezawa, D., Arakawa, K.: Functional role of winter-accumulating proteins from mulberry tree in adaptation to winter-induced stresses. In Cold Hardiness in Plants, Chen, T.H.H., Uemura, M., Fujikawa, S. (eds), CAB International, UK, p.181-202 (2006)

46. Minami, A., Nagao, M., Arakawa, K., Fujikawa, S., Takezawa, D.: Physiological and morphological alterations associated with development of freezing tolerance in the moss Physcomitrella patens. In Cold Hardiness in Plants, Chen, T.H.H., Uemura, M., Fujikawa, S. (eds), CAB International, UK, p.138-152 (2006)

47. Arakawa, K., Kasuga, J., Takashima, H., Fujikawa, S.: Physiological changes in relation to the development of freezing resistance in xylem tissue of Betula platyphylla during seasonal cold acclimation. In Tree Sap III, Terazawa,M. (ed), Hokkaido University Press, Sapporo, p.93-97 (2006)

48. Inada, H., Nagao, M., Fujikawa, S., Arakawa, K.: Influence of simulated acid snow stress on leaf tissue of wintering herbaceous plants. Plant and Cell Physiology, 47: 504-512 (2006)

49. 水野薫，春日純，荒川圭太，藤川清三: 針葉樹木部からの氷核形成阻害物質の検出. 低温生物工学会誌, 51: 111-114 (2005)

50. Takahashi, H., Arakawa, K., Fujikawa, S.: Study on proteins related to deep supercooling ability of xylem tissues of Fagus crenata L. Cryobiology and Cryotechnology, 51: 105-109 (2005)

51. Inada, H., Fujikawa, S., Arakawa, K.: Influence of the freeze-thaw process under acid conditions on the viability of wintering plants. Cryobiology and Cryotechnology, 51: 115-119 (2005)

52. Nagao, M., Minami, A., Arakawa, K., Fujikawa, S., Takezawa, D.: Rapid degradation of starch in chloroplasts and concomitant accumulation of soluble sugars associated with ABA-induced freezing tolerance in the moss Physcomitrella patens. Journal of Plant Physiology, 162: 169-180 (2005)

53. Minami, A., Nagao, M., Ikegami, K., Koshiba, T., Arakawa, K., Fujikawa, S., Takezawa, D.: Cold acclimation in bryophytes: low-temperature-induced freezing tolerance in Physcomitrella patens is associated with increases in expression levels of stress-related genes but not with increase in level of endogenous abscisic acid. Planta, 220: 414-423 (2005)

54. Ukaji, N., Kuwabara, C., Takezawa, D., Arakawa, K., Fujikawa, S.: Accumulation of pathogenesis-related (PR) 10/Bet v 1 protein homologues in mulberry (Morus bombycis Koidz.) tree during winter. Plant, Cell and Environment, 27: 1112-1121 (2004)

55. Arakawa, K., Hanazaki, M., Yoshida, S.: Frost-susceptible protein in plasma membranes in tubers of Helianthus tuberosus L. Bioscience, Biotechnology and Biochemistry, 68: 175-182 (2004)

56. Fujibe, T., Saji, H., Arakawa, K., Yabe, N., Takeuchi, Y., Yamamoto, K.T.: A methyl viologen-resistant mutant of Arabidopsis, which is allelic to ozone-sensitive rcd1, is tolerant to supplemental ultraviolet-B irradiation. Plant Physiology, 134: 275-285 (2004)

57. Mimami, A., Nagao, M., Arakawa, K., Fujikawa, S., Takezawa, D.: Abscisic acid-induced freezing tolerance in the moss Physcomitrella patens is accompanied by increased expression of stress-related genes. Journal of Plant Physiology, 160: 475-483 (2003)

58. Kuroda, K., Kasuga, J., Arakawa, K., Fujikawa, S.: Xylem ray parenchyma cells in boreal hardwood species respond to subfreezing temperatures by deep supercooling that is accompanied by incomplete desiccation. Plant Physiology, 131: 736-744 (2003)

59. Arakawa, K., Kasuga, J., Takashima, H. and Fujikawa, S.: Accumulation of extracellular proteins in birch xylem during seasonal cold acclimation. Cryobiology and Cryotechnology, 49: 195-201 (2003)

60. 荒川圭太, 稲田秀俊：キクイモ塊茎の凍結傷害過程で生じる細胞膜蛋白質の組成変化に関する研究. 低温生物工学会誌, 49: 203-207 (2003)

61. 南杏鶴, 長尾学, 荒川圭太, 藤川清三, 竹澤大輔：アブシジン酸による耐凍性上昇に伴うヒメツリガネゴケの生理的変化の解析. 低温生物工学会誌, 49: 179-183 (2003)

62. 高田直樹, 竹澤大輔, 南杏鶴, 荒川圭太, 藤川清三：深過冷却で寒冷環境に適応するカラマツ木部柔細胞の遺伝子発現. 低温生物工学会誌, 49: 191-194 (2003)

63. 春日純, 藤川清三, 荒川圭太：シラカンバ木部組織に蓄積する可溶性糖の季節変化. 低温生物工学会誌, 49: 185-189 (2003)

64. 田中聡子, 長尾学, 船田良, 藤川清三, 荒川圭太：樹木木部柔細胞の深過冷却の原因と考えられている細胞壁マイクロキャピラリーのサイズを解析する試み. 低温生物工学会誌, 49: 209-213 (2003)

65. Yamada, T., Kuroda, K., Jitsuyama, Y., Takezawa, D., Arakawa, K., Fujikawa, S.: Roles of the plasma membrane and the cell wall in the responses of plant cells to freezing. Planta, 215: 770-778 (2002)

66. Kuwabara, C., Takezawa, D., Hamada, T., Shimada, T., Fujikawa, S., Arakawa, K.: Abscisic acid- and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale. Physiologia Plantarum, 115: 101-110 (2002)

67. Ukaji, N., Kuwabara, C., Takezawa, D., Arakawa, K., Fujikawa, S.: Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis abundant proteins.

68. Plant Physiology, 126: 1588-1597 (2001)

69. Kawamura, Y., Arakawa, K., Maeshima, M., Yoshida, S.: ATP analogue binding to the A subunit induces conformational changes in the E subunit that involves a disulfide bond formation in plant V-ATPase. European Journal of Biochemistry, 268: 2801-2809 (2001)

70. Kawamura, Y., Arakawa, K., Maeshima, M., Yoshida, S.: Tissue specificity of E subunit isoforms of plant vacuolar H+-ATPase and existence of isotype enzymes. Journal of Biological Chemistry, 275: 6515-6522 (2000)

71. Fujikawa, S., Kubota, M., Kuroda, K., Takezawa, D., Arakawa, K.: Freezing adaptation mechanisms of living tissue cells in Betula platyphylla var. japonica Hara and their relation to cell wall properties. In Tree Sap II, Terazawa, M. (ed.), Hokkaido University Press, Sapporo, p.93-96 (2000)

72. Nagao, M., Arakawa, K., Takezawa, D., Yoshida, S., Fujikawa, S.: Akinete formation in Tribonema bombycinum Derbes et solier (Xanthophyceae) in relation to freezing tolerance. Journal of Plant Research, 112: 163-174 (1999)

73. 久保田勝利, 荒川圭太, 黒田克史, 藤川清三： 低温馴化によるシラカンバ木部放射柔細胞の細胞壁の変化. 低温生物工学会誌. 45: 47-50 (1999)

74. Ukaji, N., Kuwabara, C., Takezawa, D., Arakawa, K., Yoshida, S. and Fujikawa, S.: Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation. Plant Physiology, 120: 481-489 (1999)

75. Hotsubo, K., Kawamura, Y., Takezawa, D., Arakawa, K., Yoshida, S.: Characterization of vacuolar H+-ATPases that are sensitive and tolerant to cold. In Cold Hardiness in Plants, Li, P.H., Chen, T. (eds), Plenum Press, USA, p.237-244 (1998)

76. Kuwabara, C., Arakawa, K., Yoshida, S.: Abscisic acid-induced secretory proteins in suspension-cultured cells of winter wheat. Plant and Cell Physiology, 40: 184-191 (1999)

77. Koike, M., Takezawa, D., Arakawa, K., Yoshida, S.: Accumulation of 19-kDa plasma membrane polypeptide during induction of freezing tolerance in wheat suspension-cultured cells by abscisic acid. Plant and Cell Physiology, 38: 707-716 (1997)

78. Odaira, M., Arakawa, K., Yoshida, S., Maeshima, M.: Properties of 2 N-linked glycoproteins associated with the nuclear envelope in mung bean hypocotyls. Plant and Cell Physiology, 36: 945-953 (1995)

79. Zhou, B.-L., Arakawa, K., Fujikawa, S., Yoshida, S.: Cold-induced alterations in plasma membrane proteins that are specifically related to the development of freezing tolerance in cold-hardy winter wheat. Plant and Cell Physiology, 35: 175-182 (1994)